Immunofluorescence Imaging of DNA Damage and Repair

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By counting gamma-H2AX- foci, it is possible to measure very low levels of DNA damage that are inflicted during routine X-ray examinations . A comparison of our flow-cytometry method with published data indicates that foci counting can detect close to ten times lower doses of IR . Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal recombinant, expressed in HEK 293 cells, clone RM224, purified immunoglobulin Synonym: H2AX Ser139 p, Histone H2AX (phospho ser139) Keywords: Gamma-H2AX, High-content screening, Image processing, Hough transform, dosimetry 1. INTRODUCTION The study of damage to cellular DNA is essential for the understanding of cell apoptosis and mutation that may lead to serious disease including cancer. Changes in the genome caused by a single double strand break (DSB) may be enough Compare gammaH2ax ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

Gamma h2ax

Gamma H2AX foci are always formed in case of DNA double strand breaks as a first step in its repair. As there is no repair without a previous damage I would not say that these foci represent H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR, and DNA-PK (2,3). 2016-03-31 2009-08-24 Unconjugated Whole IgG Rabbit anti-gamma-H2AX Antibody, Affinity Purified suitable for WB, IHC, ICC-IF applications. Visit Bethyl.com for all your antibody needs.

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Nucleic Acids Res 36:5678–5694 PubMed CrossRef Google Scholar 2021-02-25 · p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG 1, cited in 38 publications, provided at 100 µg/ml; raised against a recombinant protein corresponding to the Ser 139 phosphorylated region of Histone H2A.X of human origin gamma-H2AX Antibody. Gene and Protein Information. H2AX is a member of the histone H2A family.

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A comparison of our flow-cytometry method with published data indicates that foci counting can detect close to ten times lower doses of IR . Compare gammaH2ax ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. Mol Cell. 2005 Dec 9;20(5):801-9. 2010-11-23 Gamma-H2AX is lost from chromatin surrounding a double-strand break independently of the HTP-C, indicating that the phosphatase targets gamma-H2AX after its displacement from DNA. The dephosphorylation of gamma-H2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. Gamma-H2AX foci in cells exposed to a mixed beam of X-rays and alpha particles Elina Staaf1*, Karl Brehwens1, Siamak Haghdoost1, Joanna Czub2 and Andrzej Wojcik1,3 Abstract Background: Little is known about the cellular effects of exposure to mixed beams of … Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139) Clone RM224 quantity Add to cart SKU: 31-1104-00 Categories: Histone H2A.Z Acetylation , Histone Modification , Primary Antibodies , Rabbit Monoclonal Tags: 31-1104-00 , 31-1104-00-S , 31110400 , 31110400S Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
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Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. Mol Cell.

Immunogen surrounding Serine 140. A700-053. Phosphorylated on Ser-140 (to form gamma-H2AX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb in the presence of control peptide (left) or Phospho-Histone H2A.X (Ser139) Blocking Peptide #1260 (right).
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Gamma H2AX foci are always formed in case of DNA double strand breaks as a first step in its repair. As there is no repair without a previous damage I would not say that these foci represent gamma-h2ax-phospho-s139-antibody-ep8542y-ab81299.pdf. Send me a copy of this email I agree to the terms and conditions. Epigenetics and Nuclear Signaling Histones Variants.

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